Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
Document Type
Article
Publication Date
11-30-2021
Abstract
As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07(T)). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H-6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster.
Keywords
Lipid-composition, Genome, Acid, Streptomyces, Identification, Classification, Sequences, Names, List, DNA
Divisions
InstituteofBiologicalSciences
Funders
Biotechnology and Biological Sciences Research Council Doctoral Training Partnership, UK (BBSRC DTP) studentship,UK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) [EP/L022494/1],University of Nottingham,Universiti Malaya [FP022-2018A]
Publication Title
PLoS ONE
Volume
16
Issue
11
Publisher
Public Library of Science
Publisher Location
1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA