Insight in the quorum sensing-driven lifestyle of the non-pathogenic agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii
Document Type
Article
Publication Date
11-1-2021
Abstract
Agrobacterium tumefaciens is considered a prominent phytopathogen, though most isolates are nonpathogenic. Agrobacteria can inhabit plant tissues interacting with other microorganisms. Yeasts are likewise part of these communities. We analyzed the quorum sensing (QS) systems of A. tumefaciens strain 6N2, and its relevance for the interaction with the yeast Meyerozyma guilliermondii, both sugarcane endophytes. We show that strain 6N2 is nonpathogenic, produces OHC8-HSL, OHC10-HSL, OC12-HSL and OHC12-HSL as QS signals, and possesses a complex QS architecture, with one truncated, two complete systems, and three additional QS-signal receptors. A proteomic approach showed differences in QS-regulated proteins between pure (64 proteins) and dual (33 proteins) cultures. Seven proteins were consistently regulated by quorum sensing in pure and dual cultures. M. guilliermondii proteins influenced by QS activity were also evaluated. Several up- and down- regulated proteins differed depending on the bacterial QS. These results show the QS regulation in the bacteria-yeast interactions.
Keywords
Quorum sensing, Interactions, Endophytic, Proteomic, Agrobacteria
Funders
Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)[0946],Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)[PU-E22920160100012CO],ANPCyT[0532],ANPCyT[2013],Secretaria de Ciencia, Arte e Innovacion Tecnologica from the Universidad Nacional de Tucuman[PIUNT D609]
Publication Title
Genomics
Volume
113
Issue
6
Publisher
Elsevier
Publisher Location
525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA