Biological and analytical investigations of alpha-glucosidase inhibitory and anti-oxidant activities on selected Malaysian medicinal plants
Document Type
Article
Publication Date
9-1-2021
Abstract
This study was performed to establish anti-diabetic and anti-oxidant properties, and to carry out phytochemical analysis of selected local plants which are traditionally used as medicinal plants. Local plants involved in this study were Lawsonia inennis, Punica granatum, Dryobalanops aromatica, Ziziphus mauritiana, and Ocimum basilicum. Solvent extraction was performed using maceration method with solvents of increasing polarity. Alpha-glucosidase inhibition assay was performed on all extracts to ascertain their anti-diabetic potentials. The extracts were screened for antioxidant activity using anti-oxidant assays (FRAP, DPPH, TAOC, ABTS, and BCB) and quantitative phytochemical analyses (TPC and TFC). Chemical profiling using LCMS and GCMS was performed on extracts with high biological activities. Methanol extracts of D. aromatica bark and leaves showed the most potent inhibition of alpha-glucosidase with IC50 values of 0.63 +/- 0.03 mu g/mL and 0.98 +/- 0.02 mu g/mL, respectively. Both extracts exhibited similar anti-oxidant activity on all five assays and possessed high phenolic contents with values of 266.79 and 261.69 GAE, respectively. The results obtained suggested that amongst the selected plants studied, D. aromatica showed high anti-oxidant activity and anti-diabetic activity (via inhibition of alpha-glucosidase). This is the first report that highlights the anti-diabetic potential of D. aromatica.
Keywords
Alpha-glucosidase, Anti-diabetic, Anti-oxidant, Dryobalanops aromatica, Medicinal plants
Divisions
Hadith,CHEMISTRY,pharchemistry
Funders
Ministry of Higher Education through the Trans Disciplinary Research Grant Scheme [TR001C-2014A],USIM C grant [PPP/UCG-0116/FPQS/30/14316]
Publication Title
Sains Malaysiana
Volume
50
Issue
9
Publisher
Penerbit Universiti Kebangsaan Malaysia
Publisher Location
FACULTY SCIENCE & TECHNOLOGY, BANGI, SELANGOR, 43600, MALAYSIA