Induction of apoptosis in Hela cells by a novel peptide from fruiting bodies of Morchella importuna via the mitochondrial apoptotic pathway
Document Type
Article
Publication Date
8-5-2021
Abstract
Morels (Morchella spp.) are a genus of edible fungi with important economic and medicinal value. In this study, a novel peptide (MIPP) was extracted from the fruiting bodies of Morchella importuna using gel filtration chromatography. Structural analysis showed that the molecular mass of MIPP is 831 Da, and it has a simple amino acid sequence: Ser-Leu-Ser-Leu-Ser-Val-Ala-Arg. To explore the antitumor activity of MIPP, the effect of MIPP on HeLa cell apoptosis and the underlying preventative mechanisms were investigated. Results showed that MIPP reduced the viability of HeLa cells in a concentration-dependent manner. TUNEL analysis and flow cytometric examination showed that MIPP decreased cell proliferation via a mitochondrial-dependent pathway, as manifested by downregulation of Bcl-2/Bax, promotion of the movement of cytochrome C from the mitochondria to the cytoplasm, and triggering of caspase-9 and caspase-3. Therefore, MIPP may be a promising tumor-preventive agent, especially in human cervical cancer.
Keywords
Cytochrome-C, Polysaccharide, Antioxidant, Antitumor, Activation, Caspase-3
Divisions
pharmacy
Funders
High-Tech Field Expansion Project of Sichuan Academy of Agricultural Sciences[2018GXTZ-001],Foundation for Subject Construction of Sichuan Academy of Agricultural Sciences[2021XKJS058],Sichuan Association for Science and Technology Young Scholar Project
Publication Title
Evidence-Based Complementary and Alternative Medicine
Volume
2021
Publisher
Hindawi
Publisher Location
ADAM HOUSE, 3RD FLR, 1 FITZROY SQ, LONDON, W1T 5HF, ENGLAND