Bioactivity profiling of Sanghuangporus lonicerinus: antioxidant, hypoglycaemic, and anticancer potential via in-vitro and in-silico approaches
Document Type
Article
Publication Date
12-1-2025
Abstract
This study investigates the mycochemical profile and biological activities of hydroethanolic (EtOH), chloroform (CHCl3), and hot water (H2O) extracts of Sanghuangporus lonicerinus from Uzbekistan. Antioxidant capacity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2 `-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), NO, and FRAP assays, and in vitro hypoglycaemic effects were evaluated through alpha-amylase and alpha-glucosidase inhibition. Antiproliferative potential was explored by analysing the binding affinities of EtOH and H2O extracts to estrogen receptor alpha (ER alpha), ER beta, androgen receptor (AR), and glucocorticoid receptor (GR), with molecular docking providing structural insights. LC-MS/MS analysis revealed solvent-dependent phenolic profiles, with the EtOH extract containing the highest total phenolic content (143.15 +/- 6.70 mg GAE/g d.w.) and the best antioxidant capacity. The EtOH extract showed significant hypoglycaemic effects, with 85.29 +/- 5.58% inhibition of alpha-glucosidase and 41.21 +/- 0.79% inhibition of alpha-amylase. Moderate ER beta binding suggests potential for estrogen-mediated cancer therapy, while strong AKR1C3 inhibition by the EtOH extract supports its therapeutic potential.
Keywords
Anticancer, Diabetes, Molecular docking, Phylogeny, Polyphenolics
Divisions
InstituteofBiologicalSciences
Funders
Agency for Innovative Development under the Ministry of Higher Education, Science, and Innovation of the Republic of Uzbekistan [Grant No: AL-8724052922-R3],Ministry of Science, Technological Development, and Innovation of the Republic of Serbia [Grant No: 451-03-66/2024-03/200125],CAS PIFI [Grant No: 2022VBA0021],Science Fund of the Jiangsu Vocational College of Agriculture and Forestry [Grant No: 2024kj07; 2023kj16]
Publication Title
Journal of Enzyme Inhibition and Medicinal Chemistry
Volume
40
Issue
1
Publisher
Taylor & Francis Ltd
Publisher Location
2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND