Matrix free laser desorption ionization coupled to trapped ion mobility mass spectrometry: an innovative approach for isomer differentiation and molecular network visualization
Document Type
Article
Publication Date
5-1-2025
Abstract
The chemical profiling of complex mixtures of natural products (NPs) is a major challenge in analytical chemistry and generally addressed by liquid chromatography coupled to mass spectrometry (LC-MS). In recent years also matrix free laser desorption ionization-mass spectrometry (LDI-MS) has become a versatile and time efficient complement to LC-MS. However, the absence of chromatographic separation in LDI-MS does not permit the differentiation of isomers. Providing a potential solution to this problem, the current work presents a combined LDI-Ion mobility spectrometry-tandem mass spectrometry (LDI-IMS-MS2) approach, which facilitated the successful differentiation of four constitutional xanthone isomers namely butyraxanthone D, cratoxylone, garcinone D and parvixanthone G. In addition, the experimental collision cross section (CCS) distribution values of nine unreported xanthones are described. Based on these results, a proof of concept for the so far unexplored concept of a LDI-IMS-MS2 based molecular network is being presented.
Keywords
Matrix free laser desorption ionization, Mass spectrometry, Ion mobility spectrometry, Isomer differentiation, Molecular network, Xanthones
Divisions
CHEMISTRY
Funders
Campus France (45708QC),Le ministere de l'Europe et des Affaires Etrangeres (MEAE),Bavarian Ministry of State for Education, Culture, Science and Art (36091TG) ; (FK-04-2022)
Publication Title
Talanta
Volume
287
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS