Matrix free laser desorption ionization coupled to trapped ion mobility mass spectrometry: an innovative approach for isomer differentiation and molecular network visualization

Document Type

Article

Publication Date

5-1-2025

Abstract

The chemical profiling of complex mixtures of natural products (NPs) is a major challenge in analytical chemistry and generally addressed by liquid chromatography coupled to mass spectrometry (LC-MS). In recent years also matrix free laser desorption ionization-mass spectrometry (LDI-MS) has become a versatile and time efficient complement to LC-MS. However, the absence of chromatographic separation in LDI-MS does not permit the differentiation of isomers. Providing a potential solution to this problem, the current work presents a combined LDI-Ion mobility spectrometry-tandem mass spectrometry (LDI-IMS-MS2) approach, which facilitated the successful differentiation of four constitutional xanthone isomers namely butyraxanthone D, cratoxylone, garcinone D and parvixanthone G. In addition, the experimental collision cross section (CCS) distribution values of nine unreported xanthones are described. Based on these results, a proof of concept for the so far unexplored concept of a LDI-IMS-MS2 based molecular network is being presented.

Keywords

Matrix free laser desorption ionization, Mass spectrometry, Ion mobility spectrometry, Isomer differentiation, Molecular network, Xanthones

Publication Title

Talanta

DOI

10.1016/j.talanta.2025.127626

Funders

Campus France (45708QC),Le ministere de l'Europe et des Affaires Etrangeres (MEAE),Bavarian Ministry of State for Education, Culture, Science and Art (36091TG) ; (FK-04-2022)

Volume

287

Publisher

Elsevier

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