Matrix free laser desorption ionization coupled to trapped ion mobility mass spectrometry: an innovative approach for isomer differentiation and molecular network visualization

Document Type

Article

Publication Date

5-1-2025

Abstract

The chemical profiling of complex mixtures of natural products (NPs) is a major challenge in analytical chemistry and generally addressed by liquid chromatography coupled to mass spectrometry (LC-MS). In recent years also matrix free laser desorption ionization-mass spectrometry (LDI-MS) has become a versatile and time efficient complement to LC-MS. However, the absence of chromatographic separation in LDI-MS does not permit the differentiation of isomers. Providing a potential solution to this problem, the current work presents a combined LDI-Ion mobility spectrometry-tandem mass spectrometry (LDI-IMS-MS2) approach, which facilitated the successful differentiation of four constitutional xanthone isomers namely butyraxanthone D, cratoxylone, garcinone D and parvixanthone G. In addition, the experimental collision cross section (CCS) distribution values of nine unreported xanthones are described. Based on these results, a proof of concept for the so far unexplored concept of a LDI-IMS-MS2 based molecular network is being presented.

Keywords

Matrix free laser desorption ionization, Mass spectrometry, Ion mobility spectrometry, Isomer differentiation, Molecular network, Xanthones

Publication Title

Talanta

Divisions

CHEMISTRY

Funders

Campus France (45708QC),Le ministere de l'Europe et des Affaires Etrangeres (MEAE),Bavarian Ministry of State for Education, Culture, Science and Art (36091TG) ; (FK-04-2022)

Volume

287

Publisher

Elsevier

Publisher Location

RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS

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