Protective effects of tiger milk mushroom extract (xLr®) against UVB irradiation in Caenorhabditis elegans via DAF-16 anti-oxidant regulation
Document Type
Article
Publication Date
1-1-2025
Abstract
Background and aim: A critical causative factor of oxidative stress and inflammation leading to several skin complications is ultraviolet-B (UVB) irradiation. Lignosus rhinocerus (LR), or tiger milk mushroom, is native to Southeast Asia. Cold water extract of an LR cultivar, TM02 (R) (xLr (R)) is a promising anti-oxidant and antiinflammatory source. However, the effects of xLr (R) on UVB-induced photoaging have never been elucidated. Experimental procedure: This study investigated the protective effects of xLr (R) and its high, medium, and low molecular weight (HLR, MLR, and LLR, respectively) fractions against UVB irradiation using in vivo Caenorhabditis elegans (C. elegans) model. Results and conclusion: The investigation revealed a significant lifespan extension of xLr (R) and its fractions in UVBirradiated C. elegans, which could be mediated by the regulation of genes associated with anti-oxidant (daf-16 and sod-3) and apoptosis (cep-1, hus-1, ced-13, and egl-1) pathways. xLr (R) significantly reduced the ROS production in C. elegans and increased the DAF-16 nuclear translocation compared to untreated worms. Additionally, the SOD-3 expression was increased in the xLr (R)-treated worms. Hence, it suggests that the different components in xLr (R) work synergistically to protect against UVB irradiation. Our findings may be beneficial for the application of xLr (R) as a treatment against UVB-induced cellular damage and photoaging.
Keywords
Ultraviolet-B, Lignosus rhinocerus, Apoptosis, Photoaging, DAF-16
Divisions
molecular
Funders
Thailand Science Research and Inno-vation Fund, Chulalongkorn University (CU_FRB65_hea (78) _173_37_03)
Publication Title
Journal of Traditional and Complementary Medicine
Volume
15
Issue
1
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS