Evolution of microbial community structure during biohydrogen production process of palm oil anaerobic sludge
Document Type
Article
Publication Date
12-1-2024
Abstract
Anaerobic microbial communities and their functions can provide information about waste management practices, as well as reveal novel degradation metabolisms that can be utilized in wastewater treatment. This study was aimed towards sustainable palm oil industry with assessing the microbial evolution during the dark fermentative processing of palm oil anaerobic sludge and palm oil mill effluent (POME) in batch-mode over twomonths with a distinct incubation time of 24 h. The process was designed using response surface methodology (RSM) by acclimatization of inoculum using POME with a chemical oxygen demand (COD) of 36 g-COD/l. The effect of mesophilic and thermophilic conditions and pH on COD removal, hydrogen content, and hydrogen yield (HY) were investigated. A potential sample was selected for microbial analysis after the first and second months of operation based on the maximum outputs achieved throughout the experiment. The bacterial community at pH 5.6 and temperature 55 degrees C with maximum hydrogen content of 64.13 %, and HY of 0.93 ml H2/g-COD was dominated by three phyla: Firmicutes, Actinobacteria, and Bacteroidota. The results obtained from high throughput pyrosequencing showed that how acclimatization period affects the microbial community structure towards Firmicutes phylum, which includes the majority of hydrogen-producing microorganisms.
Keywords
Bio-hydrogen, Dark fermentation, Palm oil mill effluent, Microbial community structure, Renewable energy
Divisions
ocean,umpedac
Funders
Swedish Energy Agency (310432),Research University of Tabriz (RU019D-2014A)
Publication Title
Renewable Energy
Volume
237
Issue
B
Publisher
Elsevier
Publisher Location
THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND