Pomegranate peel-derived punicalagin: Ultrasonic-assisted extraction, purification, and its alpha-glucosidase inhibitory mechanism

Document Type

Article

Publication Date

4-16-2022

Abstract

The pomegranate peel is a by-product of pomegranate fruit rich in polyphenols. In this study, pomegranate peel polyphenols were explored using LC-MS/MS, and punicalagin was the most abundant compound. The highest yield (505.89 +/- 1.73 mg/g DW) of punicalagin was obtained by ultrasonic-assisted extraction (UAE) with the ethanol concentration of 53%, sample-to-liquid ratio of 1:25 w/v, ultrasonic power of 757 W, and extraction time of 25 min. Punicalagin was further purified by the macroporous resin D101 and prep-HPLC, reaching the purity of 92.15%. The purified punicalagin had the IC50 of 82 +/- 0.02 mu g/mL against alpha-glucosidase, similar to the punicalagin standard with IC50 of 58 +/- 0.014 mu g/mL, both exhibiting a mixed inhibitory mechanism. Molecular docking further revealed that a steric hindrance with the intermolecular energy of - 7.99 kcal/mol was formed between punicalagin and alpha-glucosidase. Overall, pomegranate peel is a promising source of punicalagin to develop anti-diabetic functional foods.

Keywords

Polyphenols, Ultrasonic-assisted extraction, LC-MS/MS, Alpha-Glucosidase, Inhibition kinetic, Molecular docking

Publication Title

Food Chemistry

DOI

10.1016/j.foodchem.2021.131635

Divisions

medicinedept

Funders

Local Financial Funds of National Agricultural Science and Technology Center, Chengdu [NASC2020KR02],Central Public-interest Scientific Institution Basal Research Fund [Y2020XK05]

Volume

374

Publisher

Elsevier Sci Ltd

Publisher Location

THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND

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