Histological characterization of pulp regeneration using decellularized human dental pulp and mesenchymal stem cells in a feline model
Document Type
Article
Publication Date
10-1-2024
Abstract
Regenerative endodontics aims to restore pulp tissues, thus preserving the vitality of the tooth. One promising approach involves the utilization of decellularized human dental pulp (DHDP) as a scaffold repopulated with Wharton's Jelly mesenchymal stem cells (WJMSCs). This study aimed to regenerate pulp tissues using DHDP and WJMSCs following pulpectomy in mature canine teeth of a feline animal model and to investigate the histological features of the regenerated pulp. A 12-month-old male domestic shorthaired felines were used as subjects. Teeth were categorized into untreated (Group 1), pulpectomy with mineral trioxide aggregate (MTA) (Group 2), and pulpectomy with DHDP-repopulated scaffold and MTA (Group 3). The animals were sacrificed six weeks postintervention. H&E and immunohistochemistry using anti-collagen type 1 and laminin antibodies were used to stain the tissue sections. Histological examinations presented pulp-like tissues in Group 3, with tissue components similar to the structures found in Group 1. Immunohistochemical analysis demonstrated the presence of collagen type I and laminin within the regenerated tissues. The root canals of teeth in Group 2 were devoid of pulpal tissue. DHDP with WJMSCs can potentially be used for pulp regeneration, supporting the modality for developing new clinical protocols in stem cell therapy.
Keywords
Anti-collagen type 1, Immunohistochemistry, Laminin, Regenerative endodontics, Wharton's Jelly Mesenchymal Stem Cells
Divisions
Dentistry,OralMaxillofacialSurgery,restoreden
Funders
Ministry of Higher Education Malaysia (MOHE) through Fundamental Research Grant Scheme (FRGS/1/2022/SKK11/UKM/01/3)
Publication Title
Tissue and Cell
Volume
90
Publisher
Churchill Livingstone
Publisher Location
JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND