Exploring naphthoquinone and anthraquinone derivatives as antibiotic adjuvants against Staphylococcus aureus biofilms: Synergistic effects of menadione

Document Type

Article

Publication Date

10-1-2024

Abstract

Given the ability of Staphylococcus aureus to form biofilms and produce persister cells, making infections difficult to treat with antibiotics alone, there is a pressing need for an effective antibiotic adjuvant to address this public health threat. In this study, a series of quinone derivatives were evaluated for their antimicrobial and antibiofilm activities against methicillin-susceptible and methicillin-resistant S. aureus reference strains. Following analyses using broth microdilution, growth curve analysis, checkerboard assay, time-kill experiments, and confocal laser scanning microscopy, menadione was identified as a hit compound. Menadione exhibited a notable antibacterial profile (minimum inhibitory concentration, MIC = 4-16 mu g/ml; minimum bactericidal concentration, MBC = 256 mu g/ml) against planktonic S. aureus and its biofilms (minimum biofilm inhibitory concentration, MBIC50 = 0.0625-0.25 mu g/ml). When combined with oxacillin, erythromycin, and vancomycin, menadione exhibited a synergistic or additive effect against planktonic cells and biofilms of two S. aureus reference strains and six clinical isolates, highlighting its potential as a suitable adjuvant for further development against S. aureus biofilm-associated infections.

Keywords

Antimicrobial agents, Antibiofilm activity, MRSA, Naphthoquinone, Antibiotic adjuvant

Publication Title

Microbial Pathogenesis

Divisions

medbio,nanotechnology

Funders

Universiti Malaya (IIRG003-19FNW) ; (ST030-2022),Ministry of Education, Malaysia (FP070-2022)

Volume

195

Publisher

Elsevier

Publisher Location

24-28 OVAL RD, LONDON NW1 7DX, ENGLAND

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