Acquisition and evolution of the neurotoxin domoic acid biosynthesis gene cluster in Pseudo-nitzschia species

Document Type

Article

Publication Date

10-1-2024

Abstract

Of the hitherto over 60 taxonomically identified species in the genus of Pseudo-nitzschia, 26 have been confirmed to be toxigenic. Nevertheless, the acquisition and evolution of the toxin biosynthesis (dab) genes by this extensive group of Pseudo-nitzschia species remains unclear. Through constructing chromosome-level genomes of three Pseudo-nitzschia species and draft genomes of ten additional Pseudo-nitzschia species, putative genomic integration sites for the dab genes in Pseudo-nitzschia species were explored. A putative breakpoint was observed in syntenic regions in the dab gene cluster-lacking Pseudo-nitzschia species, suggesting potential independent losses of dab genes. The breakpoints between this pair of conserved genes were also identified in some dab genes-possessing Pseudo-nitzschia species, suggesting that the dab gene clusters transposed to other loci after the initial integration. A ``single acquisition, multiple independent losses (SAMIL)'' model is proposed to explain the acquisition and evolution of the dab gene cluster in Pseudo-nitzschia species. A ``single acquisition, multiple independent losses (SAMIL)'' model is proposed to explain the acquisition and evolution of the domoic acid biosynthesis gene cluster in Pseudo-nitzschia species.

Divisions

ocean

Funders

Strategic Priority Research Program of Chinese Academy of Sciences (XDB42000000) (XDB42000000),Chinese Academy of Sciences,Chinese Academy of Sciences Pioneer Hundred Talents Program,Taishan Scholar Project Special Fund (19-3-2-16-zhc),Qingdao Innovation and Creation Plan (Talent Development Program - 5th Annual Pioneer and Innovator Leadership Award),Natural Sciences and Engineering Research Council of Canada (NSERC)

Publication Title

Communications Biology

Volume

7

Issue

1

Publisher

Nature Research

Publisher Location

HEIDELBERGER PLATZ 3, BERLIN, 14197, GERMANY

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