Proteomics Studies Indicate Differential Regulation of Key Metabolism, Energy, and Stress-Responsive Proteins in Azadirachta indica Callus Upon Exposure to Salinity and Jasmonic Acid Treatments
Document Type
Article
Publication Date
9-1-2024
Abstract
Azadirachta indica or neem is recognized for its unique properties, both against insects and in improving human health. However, proteomics studies of this plant's response to abiotic stresses have yet to be reported. Application of this technique highly depends on efficient and optimized sample preparation, in deriving highly reproducible proteomics data. The present study highlights the comparison of protein extraction method which effectively recovers proteins from recalcitrant phenolic-rich tissues of neem callus. Four protocols based on TCA-acetone or/and phenol extraction methods were compared. The TCA-acetone complemented with PVPP method was the most effective approach and generated the greatest total protein yields, spot number, good resolution, and spot intensities. Subsequently, the differential proteomics performed revealed that proteins associated with oxidoreduction, energy, transcriptional, stress response, respiration, and cell division were differentially regulated in salinity stress treatment. Meanwhile, the expression of proteins classified in the group of energy, photosynthesis, protein synthesis, oxidoreduction, and post-transcriptional regulation was altered in the JA-stressed callus. The accumulation of AZA was also noticeably reduced in salinity- and JA-treated callus, suggesting these tested stress parameters were not effective in improving AZA production in the neem callus. Overall, this analysis provides valuable information on the application of plant tissue culture as an alternative for sustainable production of major bioactive constituents in A. indica. Together, the proteomics results brought forward a possible fundamental representation of the mechanism associated with salinity and JA tolerance in the neem callus.
Keywords
Azadirachta indica, Abiotic stress, Salinity, Jasmonic acid, Callus, Proteomics, Azadirachtin
Divisions
InstituteofBiologicalSciences,cebar
Funders
Universiti Malaya, Malaysia (ST009-2021),Ministry of Education, Malaysia (FP055-2017A); (FRGS/1/2017/WAB01/UM/02/2)
Publication Title
Journal of Plant Growth Regulation
Volume
43
Issue
9, SI
Publisher
Springer
Publisher Location
ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES