Evaluation of the detection method by a flotation method using a wire loop for gastrointestinal parasites
Document Type
Article
Publication Date
9-1-2024
Abstract
Infections by gastrointestinal parasites are found in a variety of animals worldwide. For the diagnosis of such infections, the flotation method is commonly used to detect parasitic microorganisms, such as oocysts or eggs, in feces. Instead of adding a flotation solution after the final centrifugation step and using a cover slip to collect the parasites, the method using a wire loop for the recovery of the organisms has been reported as one of alternative methods. However, the recovery rates of microorganisms from the flotation method have not been analysed. In the present study, the utility of a flotation method with the use of a wire loop of 8 mm in diameter (the loop method) was evaluated using different numbers of E. tenella oocysts and Heterakis gallinarum eggs, and chicken fecal samples collected at the farms. Consequently, we found that the oocysts and eggs in tubes could be collected at a ratio of 2.00 to 3.08. Thus, our results indicate that the loop method is a simple and time saving method, implicating the application for the estimated OPG/ EPG (Oocysts/Eggs per gram) of the samples.
Keywords
egg, flotation method, loop method, oocyst
Divisions
InstituteofBiologicalSciences
Funders
Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science (21K12328)
Publication Title
Veterinary Medicine and Science
Volume
10
Issue
5
Publisher
Wiley
Publisher Location
111 RIVER ST, HOBOKEN, NJ 07030 USA