Nanodiamonds and natural deep eutectic solvents as potential carriers for lipase
Document Type
Article
Publication Date
6-1-2024
Abstract
This study investigates the use of nanodiamonds (ND) as a promising carrier for enzyme immobilization and compares the effectiveness of immobilized and native enzymes. Three different enzyme types were tested, of which Rhizopus niveus lipase (RNL) exhibited the highest relative activity, up to 350 %. Under optimized conditions (1 h, pH 7.0, 40 degrees C), the immobilized ND-RNL showed a maximum specific activity of 0.765 U mg(-1), significantly higher than native RNL (0.505 U mg(-1)). This study highlights a notable enhancement in immobilized lipase; furthermore, the enzyme can be recycled in the presence of a natural deep eutectic solvent (NADES), retaining 76 % of its initial activity. This aids in preserving the native conformation of the protein throughout the reusability process. A test on brine shrimp revealed that even at low concentrations, ND-RNL had minimal toxicity, indicating its low cytotoxicity. The in silico molecular dynamics simulations performed in this study offer valuable insights into the mechanism of interactions between RNL and ND, demonstrating that RNL immobilization onto NDs enhances its efficiency and stability. All told, these findings highlight the immense potential of ND-immobilized RNL as an excellent candidate for biological applications and showcase the promise of further research in this field.
Keywords
Rhizopus niveus lipase, Natural deep eutectic solvents, Molecular dynamics
Divisions
AcademyofIslamicStudies,fac_eng,sch_che,CHEMISTRY
Funders
Sustainable Process Engineering Centre (SPEC),Ministry of Higher Education, Research and Innovation (MoHERI) Oman through TRC (BFP/RGP/EBR/22/378),International Institute for Halal Research and Training (INHART),Faculty of Engineering, University of Malaya,International Islamic University Malaysia,RU Geran-Fakulti Program University Malaya (GPF015A-2023)
Publication Title
International Journal of Biological Macromolecules
Volume
270
Issue
2
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS