Corroborative studies on chain packing characteristics of biological medium-chain-length poly-3-hydroxyalkanoates with different monomeric composition

Document Type

Article

Publication Date

6-1-2024

Abstract

Medium -chain -length poly-3-hydroxyalkanoates (mcl-PHAs) with varied monomeric compositions were biosynthesized by producer bacteria fed with different fatty acids as carbon source. Octanoic-, lauric-, stearic-, and oleic acids were used to produce four types of mcl-PHAs viz. PHA-OC, PHA -LA, PHA -ST, and PHA-OL, respectively. The mcl-PHAs as film-casted preparations exhibit distinct traits e.g., PHA-OC and PHA -ST films are less flexible than PHA -LA while PHA-OL is a sticky, glue -like material; PHA -ST is opaque whereas PHA-OC, PHA -LA, and PHA-OL displayed transparent layers. The observation is attributed to polymer chain packing and side chain crystallization. A structure -property investigation of these biopolymers was carried out employing different spectroscopic and microscopic analyses in addition to thermal analyses. Comparative analyses of the results were applied in the interpretation and discussion of structure -property relationship.

Keywords

Biological polymer, Poly-3-hydroxyalkanoate, Physicochemical characterization, Spectroscopy analyses

Divisions

Science

Funders

Ministry of Higher Education, Malaysia under the Fundamental Research Grant Scheme (FRGS/1/2020/STG01/UM/01/1),Universiti Malaya (UM.C/625/1/HIR/MOHE/05) ; (IIRG017A-2019) ; (ST021-2022)

Publication Title

International Journal of Biological Macromolecules

Volume

269

Issue

1

Publisher

Elsevier

Publisher Location

RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS

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