Phenotypic microarray characterization of hemolytic and non-hemolytic strains of Listeria monocytogenes
Document Type
Article
Publication Date
5-1-2024
Abstract
Limited information is available on the phenotypic characteristics of Listeria monocytogenes that may affect its virulence of L. monocytogenes. The study focused on comparing the differences between two strains of L. monocytogenes, one hemolytic (LLO+) and one non-hemolytic (tLLO). Using Phenotype Microarray (PM) technology, carbon, nitrogen, and phosphorus-sulfur source utilization by the strains was performed and the KEGG pathway involvement in substrate utilization was analyzed. Of the 285 substrates screened, the results suggested that D-gluconic acid and L-serine may play a role in L. monocytogenes hemolytic activity towards LLO production by generating pyruvate as an intermediate and amino acid biosynthesis, which may play an important role in virulence protein translation. D-gluconic acid and L-serine showed no effect on the tLLO strain in PM analysis and validation tests. Notably, the scope of this study was limited to these two strains of L. monocytogenes. While these findings provide valuable insights into the metabolic differences between hemolytic and nonhemolytic strains, further research is necessary to expand this understanding to a broader range of strains and explore additional factors that may influence LLO functionality.
Keywords
Listeria monocytogenes, Food safety, Listeriolysin O, Pathogenicity, Biosafety, Phenotype microarray
Divisions
InstituteofBiologicalSciences,tidrec
Funders
Ministry of Education, Malaysia (FRGS/1/2018/STG03/UM/02/12) ; (FP049-2018A),Bantuan Kecil Pendidikan (BKP) (BK056-2016),Higher Institution Center for Excellence (HICoE) program (MO002-2019)
Publication Title
LWT-Food Science and Technology
Volume
199
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS