Associations between inflammation-related LL-37 with subgingival microbial dysbiosis in rheumatoid arthritis patients
Document Type
Article
Publication Date
5-1-2022
Abstract
Objective This study investigated the subgingival microbial profile of rheumatoid arthritis (RA) patients and its associations with disease parameters and the inflammation-related antimicrobial peptide, LL-37. Methods RA and non-RA (NRA) patients were assessed for periodontal status and divided into periodontitis (CP), gingivitis (G), and healthy (H) groups. Subgingival plaque 16s rRNA gene sequencing data was processed and analyzed using the CLC Genomic Workbench (Qiagen). Bacterial diversity and co-occurrence patterns were examined. Differential abundance between groups was also investigated. Associations between bacterial genera with disease parameters and LL-37 levels were explored qualitatively using canonical correlation analysis. Results Subgingival microbial community clustered in CP status. Co-occurrence network in NRA-H was dominated by health-associated genera, while the rest of the networks' key genera were both health- and disease-associated. RA-CP displayed highly inter-generic networks with a statistically significant increase in periodontal disease-associated genera (p<0.05). In NRA-H, disease parameters and LL-37 were correlated positively with disease-associated genera while negatively with health-associated genera. However, in the remaining groups, mixed positive and negative correlations were noted with genera. Conclusion RA patients demonstrated subgingival microbial dysbiosis where the bacteria networks were dominated by health- and disease-associated genera. Mixed correlations with disease parameters and LL-37 levels were noted.
Keywords
Inflammation, LL-37, Subgingival microbial, Dysbiosis, Rheumatoid arthritis
Divisions
fac_med,medicinedept
Publication Title
Clinical Oral Investigations
Volume
26
Issue
5
Publisher
Springer Science and Business Media
Publisher Location
TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY