Effects of Drying Methods on the Antioxidant Properties of Piper betle Leaves
Document Type
Article
Publication Date
4-1-2024
Abstract
Piper betle leaf powder is increasingly utilised as a health supplement. In this study, P. betle leaves were subjected to four different drying methods: convective air-drying, oven-drying, sun-drying, and no drying, with fresh leaves as control. Their antioxidant properties were then evaluated using colourimetric assays and GC-MS. Results showed that the sun-dried leaves had the highest (p < 0.05) total antioxidant capacity (66.23 +/- 0.10 mg AAE/g), total polyphenol content (133.93 +/- 3.76 mg GAE/g), total flavonoid content (81.25 +/- 3.26 mg CE/g) and DPPH radical scavenging activity (56.48 +/- 0.11%), and the lowest alkaloid content (45.684 +/- 0.265 mg/gm). GC-MS analysis revealed that major constituents of aqueous extracts of fresh and sun-dried P. betle leaves were hydrazine 1,2-dimethyl-; ethyl aminomethylformimidate; glycerin; propanoic acid, 2-hydroxy-, methyl ester, (+/-)-; and 1,2-Cyclopentanedione. In conclusion, sun-dried leaves exhibited overall better antioxidant properties, and their aqueous extracts contained biologically active phytoconstituents that have uses in various fields.
Keywords
Piper betle, drying, total polyphenol content, total flavonoid content, DPPH radical scavenging activity, GCMS analysis
Divisions
InstituteofBiologicalSciences
Funders
University of Malaya Science Faculty
Publication Title
Molecules
Volume
29
Issue
8
Publisher
MDPI
Publisher Location
ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND