Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein Plasmodium vivax PvRBP2a
Document Type
Article
Publication Date
3-1-2024
Abstract
We previously described a novel Plasmodium vivax invasion mechanism into human reticulocytes via the PvRBP2a-CD98 receptor-ligand pair. Using linear epitope mapping, we assessed the PvRBP2a epitopes involved in CD98 binding and recognized by antibodies from patients who were infected. We identified 2 epitope clusters mediating PvRBP2a-CD98 interaction. Cluster B (PvRBP2a431-448, TAALKEKGKLLANLYNKL) was the target of antibody responses in humans infected by P vivax. Peptides from each cluster were able to prevent live parasite invasion of human reticulocytes. These results provide new insights for development of a malaria blood-stage vaccine against P vivax. Here, we identify 2 clusters of epitopes mediating the functional interaction between the Plasmodium vivax invasion ligand PvRBP2a and its host receptor CD98. Our data reveal important epitopes for development of a P vivax blood-stage vaccine.
Keywords
CD98, invasion, Plasmodium vivax, PVRBP2a, reticulocytes
Publication Title
Journal of Infectious Diseases
Recommended Citation
Tay, Matthew Zirui; Tang, Weiyi; Lee, Wenn-Chyau; Ong, Alice Soh Meoy; Novera, Wisna; Malleret, Benoit; Carissimo, Guillaume; Chacko, Ann-Marie; El-Sahili, Abbas; Lescar, Julien; Fan, Yiping; McGready, Rose M.; Chu, Cindy S.; Chan, Jerry Kok Yen; Ng, Lisa F. P.; Russell, Bruce; Nosten, Francois; and Renia, Laurent, "Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein Plasmodium vivax PvRBP2a" (2024). Research Publications (2021 to 2025). 4662.
https://knova.um.edu.my/research_publications_2021_2025/4662
Divisions
Parasit
Volume
230
Issue
3
Publisher
Oxford University Press
Publisher Location
JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA