Size Matters: Influence of Particle Size on Antioxidant, β-Glucan, and Anti-Inflammatory Potential in Pleurotus floridanus (Agaricomycetes)
Document Type
Article
Publication Date
1-1-2024
Abstract
Cellular damage resulting from elevated levels of free radicals can lead to persistent health issues. Pleurotus floridanus, an edible white oyster mushroom, is rich in beta-glucans with potent antioxidant and anti-inflamma-tory properties. In this research, we examined the beta-glucan content, total phenolic content, as well as antioxidant and anti-inflammatory potential of hot water extracts with varying particle sizes (< 75, 75-154, 154-300, and 300-600 mu m) of both whole and sliced fruiting bodies of P. floridanus. The findings revealed that the beta-glucan content increased as the particle size increased, although no significant differences were observed. Conversely, smaller particle sizes (< 75 mu m) of whole and sliced fruiting bodies of P. floridanus exhibited higher phenolic content, 2,2-diphenyl-1-picryl-hy-drazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activity, and reducing ability compared with larger particle size (> 75 mu m). Of the four samples (AW2, AW3, AS1, and AS2) with the highest antioxidant activity selected for anti-inflammatory assays, all demonstrated the ability to reduce nitric oxide and tumor necrosis factor-alpha levels, but did not enhance interleukin-10 expression in lipopolysaccharide-stimulated RAW264.7 cells. Interestingly, particle size < 75 to 300 mu m did not appear to influence the anti-inflammatory activity, because no significant differences were observed among the particle sizes. Therefore, a particle size < 300 mu m in a P. floridanushot water extract could serve as a valuable source of antioxidant and anti-inflammatory compounds to counteract the harmful effects of free radicals.
Keywords
Pleurotus floridanus, particle size, beta-glucan, antioxidant, anti-inflammatory, medicinal mushrooms
Divisions
InstituteofBiologicalSciences,pharmacy
Funders
Universiti Malaya (Project Code IIRG005A-19HWB)
Publication Title
International Journal of Medicinal Mushrooms
Volume
26
Issue
9
Publisher
Begell House
Publisher Location
50 NORTH ST, DANBURY, CT 06810 USA