Quantitative Applications of ATR-FTIR Spectroscopy with Chemometrics for the Estimation of Amikacin in Amikacin Sulphate Injections
Document Type
Article
Publication Date
1-1-2024
Abstract
Background Amikacin belongs to the class of aminoglycoside antibiotics used in the treatment of gram-negative bacterial infections. It is resistant to the aminoglycosides modifying enzymes, making it a clinically effective drug in multidrug-resistant infections.Methods In this study, a simple Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy was used for the quantification of amikacin in amikacin sulphate injection. The infrared spectra were generated in the spectral range of 4000-667 cm-1. The calibration curve was computed through TQ Analyst Pro edition software, and the partial least square regression analysis found the linearity in the range of 10-60% w/w.Results The best calibration results were obtained in the spectral region from 1040 to 1020 cm-1 with a correlation coefficient (r2) of 1.000. The residual mean standard error (RMSEC) value was 0.00235. The percent relative standard deviation (%RSD) values for intra-day and inter-day precision were less than 8.0. The percent relative error (%RE) values were calculated and found in between the range of 0.52 to 5.60. The percent recovery of the amikacin estimation was 113.09 +/- 4.27(n=3).Conclusion This validated method is considered a green method, which is suitable for the routine analysis of amikacin in amikacin sulphate injections.
Keywords
Aminoglycoside, injections, attenuated total reflectance, fourier transform infrared spectroscopy, PLS, amikacin
Divisions
pharchemistry
Funders
UCSI University Research Grant Scheme - Centre of Excellence for Research, Innovation, and Entrepreneurship (CERVIE), UCSI University, Kuala Lumpur, Malaysia (Proj-In-FPS-016)
Publication Title
Current Analytical Chemistry
Volume
20
Issue
3
Publisher
Bentham Science Publishers
Publisher Location
EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES