Platanosides from Platanus × acerifolia: New molecules, SAR, and target validation of a strong lead for drug-resistant bacterial infections and the associated sepsis
Document Type
Article
Publication Date
1-1-2024
Abstract
Three undescribed (1–3) and nine known (4–12) platanosides were isolated and characterized from a bioactive extract of the May leaves of Platanus × acerifolia that initially showed inhibition against Staphylococcus aureus. Targeted compound mining was guided by an LC-MS/MS-based molecular ion networking (MoIN) strategy combined with conventional isolation procedures from a unique geographic location. The novel structures were mainly determined by 2D NMR and computational (NMR/ECD calculations) methods. Compound 1 is a rare acylated kaempferol rhamnoside possessing a truxinate unit. 6 (Z,E-platanoside) and 7 (E,E-platanoside) were confirmed to have remarkable inhibitory effects against both methicillin-resistant S. aureus (MIC: ≤ 16 μg/mL) and glycopeptide-resistant Enterococcus faecium (MIC: ≤ 1 μg/mL). These platanosides were subjected to docking analyses against FabI (enoyl-ACP reductase) and PBP1/2 (penicillin binding protein), both of which are pivotal enzymes governing bacterial growth but not found in the human host. The results showed that 6 and 7 displayed superior binding affinities towards FabI and PBP2. Moreover, surface plasmon resonance studies on the interaction of 1/7 and FabI revealed that 7 has a higher affinity (KD = 1.72 μM), which further supports the above in vitro data and is thus expected to be a novel anti-antibacterial drug lead. © 2024 Elsevier Inc.
Keywords
Antibacterial, Enterococcus faecium, Molecular docking, Molecular ion networking (MoIN), Platanosides, Platanus × acerifolia, Staphylococcus aureus, Structure-activity relationship (SAR), Target validation
Divisions
CHEMISTRY
Funders
National Center for Complementary and Integrative Health [Grant no. RO1AT0072318-01],National Natural Science Foundation of China [Grant no. 21937002, 81773599, 82003659]
Publication Title
Bioorganic Chemistry
Volume
143
Publisher
Academic Press Inc.