The production of microbial biodiesel from cellulose-derived fungal lipid via consolidated bioprocessing
Document Type
Article
Publication Date
5-1-2023
Abstract
Lignocellulosic biomass from agro-industrial residues can potentially be used as carbon -rich carbohydrate feedstock for the conversion via oleaginous microorganisms into lipids, and subsequently microbial biodiesel. As multiple processes are required to conven-tionally convert lignocellulosic biomass into lipids, from the hydrolysis of carbohydrate into simple sugars for the fermentation, it is not economically favourable. Consolidated bioprocessing (CBP) combines the saccharification and fermentation of lignocellulosic biomass into a single processing step. As such, this study aims to screen multiple oleaginous fungi to identify the most effective lipid producers in CBP systems, followed by optimization study. Three different fungi (Aspergillus niger, Aspergillus oryzae, and Rhizopus sp.) were screened by the co-cultivation of monosaccharides. Rhizopus sp. yielded the highest lipid content. The response surface methodology (RSM) was used to optimize lipid production by Rhizopus sp. from cellulose in CBP systems. The CBP system with carbon concentration of 0.8% (w/v), temperature of 32 degrees C, and carbon to nitrogen ratio (C/N) of 99.5, yielded the optimum lipid content (51.8%). The lipid from Rhizopus sp. was then converted into biodiesel, that comprised 56.0% of saturated fatty acids. Fuel properties analysis indicated that microbial biodiesel from lipids of Rhizopus sp. is a promising alternative to diesel fuel.(c) 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords
Biodiesel, Biofuel, Consolidated bioprocess, Lignocellulosic biomass, Lipid, Oleaginous fungi
Divisions
mechanical
Funders
Ministry of Higher Education, Malaysia under Fundamental Research Grant Scheme (FRGS) [FRGS/1/2019/TK02/UIAM/02/3]
Publication Title
Environmental Technology & Innovation
Volume
30
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS