Interactomics in plant defence: Progress and opportunities

Document Type

Article

Publication Date

5-1-2023

Abstract

Interactomics is a branch of systems biology that deals with the study of protein-protein interactions and how these interactions influence phenotypes. Identifying the interactomes involved during host-pathogen interaction events may bring us a step closer to deciphering the molecular mechanisms underlying plant defence. Here, we conducted a systematic review of plant interactomics studies over the last two decades and found that while a substantial progress has been made in the field, plant-pathogen interactomics remains a less-travelled route. As an effort to facilitate the progress in this field, we provide here a comprehensive research pipeline for an in planta plant-pathogen interactomics study that encompasses the in silico prediction step to the validation step, unconfined to model plants. We also highlight four challenges in plant-pathogen interactomics with plausible solution(s) for each.

Keywords

AP-MS, Interactomics, Plant-pathogen interaction, Protein-protein interaction, Non-model plants, Y2H

Divisions

InstituteofBiologicalSciences

Funders

Kementerian Sains, Teknologi dan Inovasi,SF009-2015

Publication Title

Molecular Biology Reports

Volume

50

Issue

5

Publisher

Springer Science and Business Media B.V.

Publisher Location

VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS

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