The correlation of EMT and p53 immunohistochemical markers with cisplatin resistance in muscle invasive bladder cancer patients: A single-centred study
Document Type
Article
Publication Date
4-1-2023
Abstract
Introduction: Although epithelial-mesenchymal transition (EMT) and p53 have been established to play a pivotal role in the aggressiveness of muscle-invasive bladder cancer (MIBC), its pathological correlation to cisplatin treatment in the Malaysian patient cohort is lacking. This study aimed to evaluate the association of EMT markers, e-cadherin, vimentin and actin, as well as tumour suppressor gene, p53, in cisplatin-receiving MIBC patients. Materials and Methods: Formalin-fixed paraffinembedded (FFPE) blocks of muscle-invasive bladder cancer patients receiving cisplatin-based chemotherapy between January 2010 to December 2020 were traced. Immunohistochemistry staining was performed on traced blocks using antibodies to e-cadherin, vimentin and actin, and p53. Results: p53 and e-cadherin were stained positive in most cases (p=0.515 and 0.242 respectively), although e-cadherin showed stronger positive expression in pre-cisplatin receiving MIBC cases. All the cases stained negative for actin and vimentin except for faint staining observed in one pre-cisplatin case. Conclusion: Although this study does not show a significant correlation between EMT markers and p53 with cisplatin-responsiveness in MIBC patients, the results serve as preliminary findings on the heterogeneous outcomes of molecular staining in the Malaysian MIBC patient cohort.
Keywords
Muscle -invasive bladder cancer, epithelial-mesenchymal transition, cisplatin resistance
Divisions
pathology,pharlife
Funders
Malaysian Ministry of Higher Education: Impact -Oriented Interdisciplinary Research Grant Programme IIRG (IIRG022A-2019),Malaysian Ministry of Higher Education: Fundamental Research Grant Scheme FRGS (10770) ; (FRGS/1/2016/SKK08/UM/02/22)
Publication Title
Malaysian Journal of Pathology
Volume
45
Issue
1
Publisher
College of Pathologists, Academy of Medicine Malaysia
Publisher Location
UNIV MALAYSIA, FAC MEDICINE, DEPT PATHOLOGY, KUALA LUMPUR, 59100, MALAYSIA