Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications
Document Type
Article
Publication Date
12-1-2022
Abstract
Biphasic calcium phosphate (BCP) serves as one of the substitutes for bone as it consists of an intimate mixture of beta-tricalcium phosphate (beta-TCP) and hydroxyapatite (HAP) in different ratios. BCP, because of its inbuilt properties such as osteoconductivity, biocompatibility, and biostability in several clinical models serves as a bone substituent for orthopedic applications. Therefore, the present study aimed to assess the effectiveness of silver (Ag) nanoparticles (NPs) combined with BCP composites for the orthopedic sector of bone tissue regeneration and growth. In this regard, we first synthesized Ag-BCP microclusters by the double-emulsion method and then characterized the composite for various physicochemical properties, including the crystallinity and crystal structure, bonding and functionality, porosity, morphology, surface charges, topography, and thermal stability. In addition, the antibacterial activity of Ag-BCP was tested against gram-positive and gram-negative microorganisms such as Staphylococcus aureus, Candida albicans, and Escherichia coli. Finally, the cytocompatibility of Ag-BCP was confirmed against the fibroblast cells in vitro.
Keywords
Biphasic calcium phosphate, Ag nanoparticles, Antibacterial activity, Hydroxyapatite, Beta-tricalcium phosphate, MTT assay
Divisions
nanocat
Funders
Council of Scientific & Industrial Research (CSIR) - India (Grant No: 09/115(0793)/2020 EMR-I)
Publication Title
Antibiotics-Basel
Volume
11
Issue
12
Publisher
MDPI
Publisher Location
ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND