Aptamer-17 beta-estradiol-antibody sandwich ELISA for determination of gynecological endocrine function

Document Type

Article

Publication Date

8-1-2021

Abstract

17 beta-Estradiol-E2 (17 beta-E2) is a steroid hormone that plays a major role in the reproductive endocrine system and is involved in various processes, such as pregnancy, fertility, and menopause. In this study, the performance of an enzyme-linked immunosorbent assay (ELISA) for 17 beta-E2 quantification was enhanced by using a gold nanoparticle (GNP)-conjugated aptamer. An anti-17 beta-E2-aptamer-GNP antibody was immobilized on an amine-modified ELISA surface. Then, 17 beta-E2 was allowed to interact with and be sandwiched by antibodies. Aptamer-GNP conjugation was confirmed by colorimetric assays via the naked eye and UV-visible light spectroscopy. The detection limit based on a signal-to-noise ratio (S/N) of 3 was estimated to be 1.5 nM (400 pg/mL), and the linear range was 1.5-50 nM. Control experiments (without 17 beta-E2/with a complementary aptamer sequence/with a nonimmune antibody) confirmed the specific detection of 17 beta-E2. Moreover, 17 beta-E2 spiking of human serum did not interrupt the interaction between 17 beta-E2 and its antibody and aptamer. Thus, the developed ELISA can be used as an alternate assay for quantification of 17 beta-E2 and assessment of endocrine-related gynecological problems. (c) 2020 International Union of Biochemistry and Molecular Biology, Inc.

Keywords

Aptamer, 17 beta-Estradiol, ELISA, Gold nanoparticle, Gynecological endocrine

Publication Title

Biotechnology and Applied Biochemistry

Divisions

Dentistry

Volume

68

Issue

4

Publisher

Wiley

Publisher Location

111 RIVER ST, HOBOKEN 07030-5774, NJ USA

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