Culturable bacteria in adults of a Southeast Asian black fly, Simulium tani (Diptera:Simuliidae)
Document Type
Article
Publication Date
7-1-2021
Abstract
Although the microbiome of blood-feeding insects serves an integral role in host physiology, both beneficial and pathogenic, little is known of the microbial community of black flies. An investigation, therefore, was undertaken to identify culturable bacteria from one of Malaysia's most common black flies, Simulium tani Takaoka and Davies, using 16S rDNA sequencing, and then evaluate the isolates for antibiotic resistance and virulence genes. A total of 20 isolates representing 11 bacterial species in four genera were found. Five isolates showed beta-hemolysis on Columbia agar, and virulence genes were found in three of these isolates. Some degree of resistance to six of the 12 tested antibiotics was found among the isolates. The baseline data from this study suggest rich opportunities for comparative studies exploring the diversity and roles of the microbiome of S. tani and other Southeast Asian black flies.
Keywords
Antibiotic resistance, Bacillus, Microbiome, Virulence genes, Vector, Infecious-diseases
Divisions
fac_med
Funders
Ministry of Higher Education, Malaysia under the Higher Institution Centre of Excellence (HICoE) program[MO002-2019]
Publication Title
Acta Tropica
Volume
219
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS