Impact of early biofilm on the assessment of initial enamel erosion with swept-source optical coherence tomography
Document Type
Article
Publication Date
6-1-2021
Abstract
Objective: This study examined the impact of early biofilm on the tooth surface, during the assessment of initial enamel erosion using swept-source optical coherence tomography (SS-OCT). Method and materials: Forty-five enamel windows of 2 x 4 mm(2) were prepared on 23 extracted human teeth. The specimens were exposed to citric acid (pH 3.2) for 30 minutes and randomly divided into three groups (n = 15): Group 1, no biofilm; Group 2, 1- day-old biofilm; and Group 3, 3-day-old biofilm. Specimens in Groups 2 and 3 were inoculated with oral bacteria (Streptococcus sanguinis, Streptococcus mitis, and Actinomyces naeslundii) to produce early laboratory-cultivated biofilms for 1 and 3 days respectively. Surface microhardness (SMH) measurements were taken at pre- (t(1)) and post-erosion (t(2)); and SS-OCT scans were done at t(1), t(2), and post-biofilm cultivation (t(3)). Integrated reflectivity (IR) of the tooth-air interface (IRsurface) and enamel (IRenamel) were computed from the mean A-scans. Statistical analysis was performed using paired t tests and one-way ANOVA (a =.05). Results: A significant increase in IRenamel was observed at t(2) (P <.05). At t(3), IRsurface between Group 1 (control) and Group 2 (P =.012) as well as Group 3 (P =.001) were significantly different. Significant variances in IR(ename)l were perceived between t(2) and t(3) for Groups 2 and 3 but not for Group 1. Conclusion: As early biofilm affected SS-OCT assessment of initial enamel erosion, they should be removed from the tooth surface prior to OCT procedures.
Keywords
Assessment, Biofilm, Detection, Enamel erosion, Initial erosion, Optical coherence tomography
Divisions
Dentistry
Funders
Universiti Malaya,High Impact Research Grant, Ministry of Higher Education, Malaysia (UM-MOHE HIR Grant) [UM.C/HIR/MOHE/DENT/11] [H-18001-00-C000011]
Publication Title
Quintessence International
Volume
52
Issue
6
Publisher
Quintessence Publ Co Inc,
Publisher Location
4350 CHANDLER DRIVE, HANOVER PARK, IL 60133 USA