Ternary glycerol-based deep eutectic solvents: Physicochemical properties and enzymatic activity
Document Type
Article
Publication Date
5-1-2021
Abstract
The present study investigates deep eutectic solvents (DESs) as potential media for enzymatic hydrolysis. A series of ternary ammonium and phosphonium-based DESs were prepared at different molar ratios by mixing with aqueous glycerol (85%). The physicochemical properties including surface tension, conductivity, density, and viscosity were measured at a temperature range of 298.15 K - 363.15 K. The eutectic points were highly influenced by the variation of temperature. The eutectic point of the choline chloride: glycerol: water (ratio of 1: 2.55: 2.28) and methyltriphenylphosphonium bromide:glycerol:water (ratio of 1: 4.25: 3.75) is 213.4 K and 255.8 K, respectively. The stability of the lipase enzyme isolated from porcine pancreas (PPL) and Rhizopus niveus (RNL) toward hydrolysis in ternary DESs medium was investigated. The PPL showed higher activity compared to the RNL in DESs. Molecular docking simulation of the selected DES with the substrate (p-nitrophenyl palmitate) toward PPL was also reported. It is worth noting that ternary DES systems would be viable lipase activators in hydrolysis reactions. (c) 2021 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
Keywords
Pancreatic lipase, Green solvent, Rhizopus niveus lipase, Ionic liquid, Choline chloride, Molecular docking simulation, Enzymatic hydrolysis
Divisions
Hadith,sch_che,CHEMISTRY
Funders
University of Malaya RU Geran Fakulti Program [GPF040A2018],University of Malaya Grant Bantuan Kecil Penyelidikan (BKP) [BK033-2017]
Publication Title
Chemical Engineering Research & Design
Volume
169
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS