Integrative microbiomics in bronchiectasis exacerbations
Document Type
Article
Publication Date
4-1-2021
Abstract
Bronchiectasis, a progressive chronic airway disease, is characterized by microbial colonization and infection. We present an approach to the multi-biome that integrates bacterial, viral and fungal communities in bronchiectasis through weighted similarity network fusion (https://integrative-microbiomics.ntu.edu.sg). Patients at greatest risk of exacerbation have less complex microbial co-occurrence networks, reduced diversity and a higher degree of antagonistic interactions in their airway microbiome. Furthermore, longitudinal interactome dynamics reveals microbial antagonism during exacerbation, which resolves following treatment in an otherwise stable multi-biome. Assessment of the Pseudomonas interactome shows that interaction networks, rather than abundance alone, are associated with exacerbation risk, and that incorporation of microbial interaction data improves clinical prediction models. Shotgun metagenomic sequencing of an independent cohort validated the multi-biome interactions detected in targeted analysis and confirmed the association with exacerbation. Integrative microbiomics captures microbial interactions to determine exacerbation risk, which cannot be appreciated by the study of a single microbial group. Antibiotic strategies probably target the interaction networks rather than individual microbes, providing a fresh approach to the understanding of respiratory infection.
Keywords
Bronchiectasis, Chronic airway disease, Respiratory infection
Publication Title
Nature Medicine
Recommended Citation
Mac Aogain, Micheal; Narayana, Jayanth Kumar; Tiew, Pei Yee; Ali, Nur A'tikah Binte Mohamed; Yong, Valerie Fei Lee; Jaggi, Tavleen Kaur; Lim, Albert Yick Hou; Keir, Holly R.; Dicker, Alison J.; Thng, Kai Xian; Tsang, Akina; Ivan, Fransiskus Xaverius; Poh, Mau Ern; Oriano, Martina; Aliberti, Stefano; Blasi, Francesco; Low, Teck Boon; Ong, Thun How; Oliver, Brian; Giam, Yan Hui; Tee, Augustine; Koh, Mariko Siyue; Abisheganaden, John Arputhan; Tsaneva-Atanasova, Krasimira; Chalmers, James D.; and Chotirmall, Sanjay H., "Integrative microbiomics in bronchiectasis exacerbations" (2021). Research Publications (2021 to 2025). 11809.
https://knova.um.edu.my/research_publications_2021_2025/11809
Divisions
fac_med
Funders
Ministry of Health-Singapore National Medical Research Council, Singapore [Grant No: NMRC/TA/0048/2016],Clinician-Scientist Individual Research Grant [Grant No: MOH-000141],NTU Integrated Medical, Biological and Environmental Life Sciences (NIMBELS) [Grant No: NIM/03/2018 ],British Lung Foundation through the GSK/British Lung Foundation Chair of Respiratory Research,Scottish Government Chief Scientist Office [Grant No: SCAF/17/03],UK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) [Grant No: EP/N014391/1]
Volume
27
Issue
4
Publisher
Nature Portfolio
Publisher Location
HEIDELBERGER PLATZ 3, BERLIN, 14197, GERMANY