Antioxidant activity of Litsea petiolata Hk. f
Document Type
Conference Item
Publication Date
4-1-2021
Abstract
Litsea petiolata Hk. f was included Lauraceae family, and the previous study had been isolated 5 compounds from the Litsea petiolata Hk. f stem bark dichloromethane extract namely harman or aribine, norharman, reticuline, isoboldine, and thalifoline. Antioxidants can prevent tissue damage by free radical. Free radical production continuously in all cells as cellular function usually, but excess production can cause many diseases. The research aimed to assay the activity of antioxidant from the extract and fractions of the Litsea petiolata Hk. f stem bark with DPPH assay and FRAP assay. The extract was obtained by soxhletation used dichloromethane as solvent. The fractions fractionated with column chromatography. The antioxidant test used DPPH assay and FRAP assay. The IC50 values for the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test of the dichloromethane extract was 27.36 µg/mL, the fraction A was 113.74 µg/mL, fraction B was 60.17 µg/mL, and the control positive (quercetin) was 3.96 µg/ml. The EC50 values for ferric ion reducing antioxidant potential (FRAP) test of the dichloromethane extract was obtained 13.47 µg/mL, the fraction A was 76.49 µg/mL, fraction B was 55.73 µg/mL, and the control positive (quercetin) was 14.01 µg/ml. The extract had higher antioxidant activity than the fractions, and by FRAP test the extract showed better antioxidant activity than the positive control (quercetin). © Published under licence by IOP Publishing Ltd.
Keywords
Antioxidants, Free radical, Tissue damage, Dichloromethane solvent, Ferric ion
Divisions
chemistry
Funders
Kementerian Riset, Teknologi dan Pendidikan Tinggi [Grant No: 26 / SP2H / DRPM / LPPM / III / 2020]
Publication Title
Journal of Physics: Conference Series
Volume
1869
Issue
1
Event Title
2nd Annual Conference of Science and Technology, ANCOSET 2020
Event Location
Malang, Virtual
Event Dates
28 November 2020
Event Type
conference