Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential
Document Type
Article
Publication Date
3-15-2021
Abstract
Background: Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of Streptomyces spp. against Plasmodium falciparum 3D7 by using a metabolomics approach. Methods: Streptomyces strains' growth curves, namely SUK 12 and SUK 48, were measured and P. falciparum 3D7 IC50 values were calculated. Metabolomics analysis was conducted on both strains' mid-exponential and stationary phase extracts. Results: The most successful antiplasmodial activity of SUK 12 and SUK 48 extracts shown to be at the stationary phase with IC50 values of 0.8168 ng/mL and 0.1963 ng/mL, respectively. In contrast, the IC50 value of chloroquine diphosphate (CQ) for antiplasmodial activity was 0.2812 ng/mL. The univariate analysis revealed that 854 metabolites and 14, 44 and three metabolites showed significant differences in terms of strain, fermentation phase, and their interactions. Orthogonal partial least square-discriminant analysis and S-loading plot putatively identified pavettine, aurantioclavine, and 4-butyldiphenylmethane as significant outliers from the stationary phase of SUK 48. For potential isolation, metabolomics approach may be used as a preliminary approach to rapidly track and identify the presence of antimalarial metabolites before any isolation and purification can be done.
Keywords
Streptomyces, Anti-plasmodial, Plasmodium falciparum, Metabolomics, Multivariate analysis
Divisions
pathology
Funders
Science and Technology Development Fund (STDF) Ministry of Higher Education & Scientific Research (MHESR) [FRGS/1/2016/STG05/UKM/02/5]
Publication Title
PeerJ
Volume
9
Publisher
PeerJ
Publisher Location
341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND