Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency
Document Type
Article
Publication Date
3-1-2021
Abstract
Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.
Keywords
Proton pump inhibitor, Endosome, Bacteria DNA delivery, Lysosome, V-ATPase, Endocytosed
Divisions
InstituteofBiologicalSciences
Funders
MOSTI eScience grant from the Ministry of Science, Technology, and Innovation, Malaysia (02-02-10-SF0252),Monash University
Publication Title
Analytical Biochemistry
Volume
616
Publisher
Academic Press Inc Elsevier Science
Publisher Location
525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA