In vitro and in vivo evaluation of a nanofiber wound dressing loaded with melatonin
Document Type
Article
Publication Date
3-1-2021
Abstract
Wound healing is a complicated process that takes a long time to complete. The three-layer nanofiber wound dressing containing melatonin is highly expected to show remarkable wound repair by reducing the wound healing time. In this study, chitosan (Cs)-polycaprolactone (PCL)/ polyvinylalcohol (PVA)-melatonin (MEL)/ chitosan-polycapmlactone three-layer nanofiber wound dressing was prepared by electrospinning for melatonin sustained release. The characteristics of the wound dressing were further evaluated. The wound dressing had a high water uptake after 24 h (401%), and the water contact angle results showed that it had hydrophilicity effect that supported the cell attachment. The wound healing effect of wound dressing was examined using a full-thickness excisional model of rat skin by the local administration of MEL. The gene expressions of transforming growth factor-beta (TGF-beta 1), alpha-smooth muscle actin (alpha-SMA), collagen type I (COL1A1), and collagen type III (COL3A1) were further studied. The histopathological evaluation showed the complete regeneration of the epithelial layer, remodeling of wounds, collagen synthesis, and reduction in inflammatory cells. The NF + 20% MEL significantly increased TGF-beta 1, COL1A1, COL3A1, and alpha-SMA mRNA expressions. This wound dressing may have a considerable potential as a wound dressing to accelerate the wound healing.
Keywords
Melatonin, Drug delivery, Nanofiber wound dressing, Polycaprolactone, Polyvinyl alcohol, Chitosan, Electrospinning
Divisions
fac_med
Funders
University of Tehran (18/06/28445)
Publication Title
International Journal of Pharmaceutics
Volume
596
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS