Lysine modification of human serum albumin and its effect on protein conformation and nalidixic acid binding
Document Type
Article
Publication Date
3-1-2021
Abstract
In order to investigate the involvement of lysine residues of human serum albumin (HSA) in nalidixic acid (NA) binding, various modified preparations of HSA such as 44% carbamylated (C-44), 83% carbamylated (C-83) and 85% acetylated (A(85)) were made by treating the HSA solution with a different molar excess of potassium cyanate and acetic anhydride. The extent of modification, charge homogeneity and conformational changes of these derivatives were checked by TNBSA reaction method, polyacrylamide gel electrophoresis (PAGE) and gel filtration using Sephacryl S-200 HR column, respectively. Binding of NA to HSA and its derivatives was examined using fluorescence quenching titration method to determine the binding constant. The emergence of a single band in PAGE and single symmetrical peak in gel filtration results confirmed the charge and size homogeneity of these derivatives. Hydrodynamic properties such as Stokes radius and frictional ratio, as obtained from the analytical gel filtration results suggested molecular expansion in C-83 and A(85) HSAs while C-44 HSA retained the native conformation. Addition of NA to both native and modified HSA derivatives quenched the fluorescence intensity of the protein at 344 nm to a different extent. Whereas the values of the Stern-Volmer constant (K-SV) and bimolecular quenching rate constant (k(q)) suggested, NA-HSA complex formation, binding constant (K-a) value suggested an intermediate binding affinity between NA and HSA. Furthermore, the decrease in the K-a value with the extent of modification was indicative of the involvement of lysine residues in NA-HSA interaction.
Keywords
Chemical modification, Human serum albumin, Drug-protein interaction, Nalidixic acid
Divisions
Dentistry
Funders
Universiti Malaya (RG012/09AFR)
Publication Title
Journal of the Indian Chemical Society
Volume
98
Issue
3
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS