Construction of a microRNA-mRNA regulatory network in de novo cytogenetically normal acute myeloid leukemia patients
Document Type
Article
Publication Date
3-1-2021
Abstract
Background: The association between dysregulated microRNAs (miRNAs) and acute myeloid leukemia (AML) is well known. However, our understanding of the regulatory role of miRNAs in the cytogenetically normal AML (CN-AML) subtype pathway is still poor. The current study integrated miRNA and mRNA profiles to explore novel miRNA-mRNA interactions that affect the regulatory patterns of de novo CN-AML. Methods: We utilized a multiplexed nanoString nCounter platform to profile both miRNAs and mRNAs using similar sets of patient samples (n = 24). Correlations were assessed, and an miRNA-mRNA network was constructed. The underlying biological functions of the mRNAs were predicted by gene enrichment. Finally, the interacting pairs were assessed using TargetScan and microT-CDS. We identified 637 significant negative correlations (false discovery rate <0.05). Results: Network analysis revealed a cluster of 12 miRNAs representing the majority of mRNA targets. Within the cluster, five miRNAs (miR-495-3p, miR-185-5p, let-7i-5p, miR-409-3p, and miR-127-3p) were posited to play a pivotal role in the regulation of CN-AML, as they are associated with the negative regulation of myeloid leukocyte differentiation, negative regulation of myeloid cell differentiation, and positive regulation of hematopoiesis. Conclusion: Three novel interactions in CN-AML were predicted as let-7i-5p:HOXA9, miR-495-3p:PIK3R1, and miR-495-3p:CDK6 may be responsible for regulating myeloid cell differentiation in CN-AML.
Keywords
acute myeloid leukemia, hematological malignancies, miRNA, mRNA, interaction
Publication Title
Genetic Testing And Molecular Biomarkers
Recommended Citation
Esa, Ezalia; Hashim, Ariwibawa Kasmani; Mohamed, Elsa Haniffah Mejia; Zakaria, Zubaidah; Abu Hassan, Alifah Nadia; Yusoff, Yuslina Mat; Kamaluddin, Nor Rizan; Rahman, Ahmad Zuhairi Abdul; Chang, Kian-Meng; Mohamed, Rashidah; Subbiah, Indhira; Jamian, Ehram; Ho, Caroline Siew-Ling; Lim, Soo-Min; Lau, Peng-Choon; Pung, Yuh-Fen; and Zain, Shamsul Mohd, "Construction of a microRNA-mRNA regulatory network in de novo cytogenetically normal acute myeloid leukemia patients" (2021). Research Publications (2021 to 2025). 11504.
https://knova.um.edu.my/research_publications_2021_2025/11504
Divisions
fac_med
Funders
Fundamental Research Grant Scheme [FP027-2018A]
Volume
25
Issue
3
Publisher
Mary Ann Liebert, Inc
Publisher Location
140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA