Novel live cell fluorescent probe for human-induced pluripotent stem cells highlights early reprogramming population
Document Type
Article
Publication Date
2-5-2021
Abstract
Background Despite recent rapid progress in method development and biological understanding of induced pluripotent stem (iPS) cells, there has been a relative shortage of tools that monitor the early reprogramming process into human iPS cells. Methods We screened the in-house built fluorescent library compounds that specifically bind human iPS cells. After tertiary screening, the selected probe was analyzed for its ability to detect reprogramming cells in the time-dependent manner using high-content imaging analysis. The probe was compared with conventional dyes in different reprogramming methods, cell types, and cell culture conditions. Cell sorting was performed with the fluorescent probe to analyze the early reprogramming cells for their pluripotent characteristics and genome-wide gene expression signatures by RNA-seq. Finally, the candidate reprogramming factor identified was investigated for its ability to modulate reprogramming efficiency. Results We identified a novel BODIPY-derived fluorescent probe, BDL-E5, which detects live human iPS cells at the early reprogramming stage. BDL-E5 can recognize authentic reprogramming cells around 7 days before iPS colonies are formed and stained positive with conventional pluripotent markers. Cell sorting of reprogrammed cells with BDL-E5 allowed generation of an increased number and higher quality of iPS cells. RNA sequencing analysis of BDL-E5-positive versus negative cells revealed early reprogramming patterns of gene expression, which notably included CREB1. Reprogramming efficiency was significantly increased by overexpression of CREB1 and decreased by knockdown of CREB1. Conclusion Collectively, BDL-E5 offers a valuable tool for delineating the early reprogramming pathway and clinically applicable commercial production of human iPS cells.
Keywords
DOFLA library fluorescence dye, Human induced pluripotent stem cell (hiPSC), Early stage pluripotency, Mesenchymal-epithelial transition (MET), Adipose-derived stromal cell (ASC), Dental pulp stem cell (DPSC), Golgi marker, Three-dimensional (3D) microcarrier-based culture system, cAMP responsive element binding protein (CREB)
Divisions
Dentistry
Funders
Development Programme Grant by A*STAR's Joint Council Office [1334 k00083],Ministry of Education, Malaysia [UM.C/HIR/MOHE/DENT/01] [UMRG RP019/13HTM]
Publication Title
Stem Cell Research and Therapy
Volume
12
Issue
1
Publisher
BioMed Central
Publisher Location
CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND