Raman spectroscopy as process analytical tool in downstream processing of biotechnology
Document Type
Article
Publication Date
2-1-2021
Abstract
Downstream processing or product recovery plays a vital role in the development of bioprocesses. To improve the bioprocess efficiency, some unconventional methods are much required. The continuous manufacturing in downstream processing makes the Process Analytical Technologies (PATs) as an important tool. Monitoring and controlling bioprocess are an essential factor for the principles of PAT and quality by design. Spectroscopic methods can apply to monitor multiple analytes in real-time with less sample processing with significant advancements. Raman spectroscopy is an extensively used technique as an analytical and research tool owing to its modest process form, non-destructive, non-invasive optical molecular spectroscopic imaging with computer-based analysis. Generally, its application is essential for the analysis and characterization of biological samples, and it is easy to operate with minimal sample. The innovation on various types of enhanced Raman spectroscopy was designed to enhance the Raman analytical technique. Raman spectroscopy could couple with chemometrics to provide reliable alternative analysis method of downstream process analysis. Thus, this review aims to provide useful insight on the application of Raman spectroscopy for PAT in downstream processing of biotechnology and Raman data analysis in biological fields. (C) 2020 The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights reserved.
Keywords
Raman spectroscopy, Process analytical technology, Downstream bioprocessing, Biotechnology
Divisions
InstituteofBiologicalSciences
Funders
National Natural Science Foundation of China (NSFC) (21878263),National Natural Science Foundation of China (NSFC) (22078286),Zhejiang University,Talent-Introduction Program of China
Publication Title
Chinese Journal of Chemical Engineering
Volume
30
Publisher
Chinese Journal of Chemical Engineering
Publisher Location
NO 13, QINGNIANHU SOUTH ST, DONGCHENG DIST, BEIJING 100011, PEOPLES R CHINA