Laser-heated needle for biopsy tract ablation: In vivo study of rabbit liver biopsy
Document Type
Article
Publication Date
2-1-2021
Abstract
To investigate the efficacy of a newly-developed laser-heated core biopsy needle in the thermal ablation of biopsy tract to reduce hemorrhage after biopsy using in vivo rabbit's liver model. Materials and methods: Five male New Zealand White rabbits weighed between 1.5 and 4.0 kg were anesthetized and their livers were exposed. 18 liver biopsies were performed under control group (without tract ablation, n 9) and study group (with tract ablation, n = 9) settings. The needle insertion depth (similar to 3 cm) and rate of retraction (similar to 3 mm/s) were fixed in all the experiments. For tract ablation, three different needle temperatures (100, 120 and 150 degrees C) were compared. The blood loss at each biopsy site was measured by weighing the gauze pads before and after blood absorption. The rabbits were euthanized immediately and the liver specimens were stained with hematoxylin-eosin (H&E) for further histopathological examination (HPE). Results: The average blood loss in the study group was reduced significantly (p < 0.05) compared to the control group. The highest percentage of bleeding reduction was observed at the needle temperature of 150 degrees C (93.8%), followed by 120 degrees C (85.8%) and 100 degrees C (84.2%). The HPE results show that the laser-heated core biopsy needle was able to cause lateral coagulative necrosis up to 14 mm diameter along the ablation tract. Conclusion: The laser-heated core biopsy needle reduced hemorrhage up to 93.8% and induced homogenous coagulative necrosis along the ablation tract in the rabbits' livers. This could potentially reduce the risk of tumor seeding in clinical settings.
Keywords
Biopsy, Tract ablation, Fiber laser, Hemorrhage, Coagulative necrosis, Rabbits
Divisions
fac_med
Funders
Prototype Research Grant Scheme, Ministry of Education Malaysia[PRGS/1/2017/SKK03/UM/02/1],Taylor's University through the Taylor's Research Scholarship Programme
Publication Title
Physica Medica-European Journal of Medical Physics
Volume
82
Publisher
Elsevier
Publisher Location
THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND