Microbial fuel cell-based sensor for Enterobacter sp. KBH6958 activity monitoring during hydrogen production: The effects of pH and glucose concentration
Document Type
Article
Publication Date
9-1-2022
Abstract
A microbial fuel cell (MFC) is an electricity-generating device utilising electrochemically active microorganisms as biocatalysts. Using MFC as a biosensor to monitor hydrogen fermentation in Enterobacter sp. is possible because the device depends on microbial activity for electricity output. This study aimed to prove the concept that voltage signal generated by Enterobacter sp. via an MFC can be used in the fast detection and monitoring of microbial health in terms of hydrogen production and microbial growth. The anodic chamber of a two-chamber MFC was used for cultivating culture and hydrogen production. An anode inside the chamber was used as a sensor terminal to monitor fermentative batch operation within 24 h. The open-circuit voltage (OCV) signal was proportional to the dried weight biomass and accumulated biogas volume (sigmoid curve) between the lag and early stationary phases. However, the OCV value started to decrease dramatically when the pH was significantly dropped during the stationary phase until the end of the experiments. The observation indicated that the pH had a great effect not only on biomass growth and biogas production but also on the OCV output. The results showed that the MFC-based health sensor is applicable, can be the cost-effective and facilitates the monitoring of the hydrogen fermentative process. GRAPHICS] .
Keywords
Microbial fuel cell sensor, Enterobacter sp., Biomass growth, Biohydrogen production, pH effect, Glucose concentration
Divisions
sch_che
Funders
Ministry of Education, Malaysia (Grant No: FRGS/1/2020/STG05/UKM/03/2),Universiti Kebangsaan Malaysia (Grant No: GGPM-2019-028 & PP-SELFUEL-2021),Natural Resources Malaysia via Tin Industry (Research and Development) Board (Grant No: RA-2021-001),Ministry of Energy
Publication Title
Journal of Applied Electrochemistry
Volume
52
Issue
9
Publisher
Springer
Publisher Location
VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS