Viability of bovine opu-derived oocytes to honeybee as cryoprotectant
Document Type
Conference Item
Publication Date
1-1-2021
Abstract
This study designs to determine the effectiveness of Honeybee (HB) as cryoprotectants (CP) on viability vitrified-thawed bovine oocytes derived from OPU using Trehalose as control. Cattles were subjected to superstimulation protocol, per session conducted five days where three days both cattle were administrated 100 mg follicle stimulation hormone (FSH) within 24 h once and two days of "resting period" totalling two sessions. The "coasting period" (FSH starvation) between sessions was four days (96 h). Oocytes collection via OPU were performed at fifth day (120 h). The ovarian growth was observed via ultrasonographic before OPU. Prior to vitrifying oocytes with treatment Trehalose (T1) and HB (T2) followed by warming protocol, oocytes subjected to in vitro maturation (IVM). Oocytes viability were evaluated by fluorescein diacetate staining. Results showed ovarian growth for first session was larger size follicles than the second session for both cattle. Total number of oocytes obtained were 60. Oocytes viability treatment T2 was significantly higher (90.9) than T1 (70.4). This study concludes that HB as CP in vitrification protocol was able to achieved high oocytes viability with oocytes derived via OPU suggesting Honeybee as an alternative CP for oocytes vitrification. © 2021 Institute of Physics Publishing. All rights reserved.
Keywords
Cattles, Cryoprotectants, Fluorescein diacetate, In-vitro, Resting period, Study design, Superstimulation
Divisions
biology
Funders
None
Publication Title
IOP Conference Series: Earth and Environmental Science
Volume
756
Issue
1
Event Title
3rd Asia Pacific Regional Conference on Food Security, ARCoFS 2021
Event Location
Kelantan, Virtual
Event Dates
9 March 2021
Event Type
conference