PEGylated DPPC/Anti-SNAP25 antibody targeted liposomes from langmuir monolayer study to formulations
Document Type
Article
Publication Date
1-1-2021
Abstract
Background: Molecule compatibility is an important factor to be considered before pre-paring antibody-targeted liposomes, stealth-liposomes, and stealth antibody-targeted liposomes. Objective: To determine the intermolecular interaction of 1,2-dioleoyl-sn-glycero-3-phosphoethano-lamide-N-methoxy(polyethyleneglycol)-2000 (ammonium salt), DOPE PEG2000 and Anti-S-NAP25 (AS25) in 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine (DPPC) monolayer, and their li-posomes. Methods: In this study, DPPC was used to create a monolayer mimicking the half membrane of li-posomes to investigate its interactions with a polyclonal antibody, AS25, and DOPE PEG2000, which are based on Langmuir-Blodgett (LB) techniques. The surface morphology of DPPC-AS25 and DPPC-DOPE PEG2000-AS25 bilayers were also imaged and analyzed by using atomic force microscopy (AFM) to support the LB findings. The LB findings were then utilized as a reference to prepare DPPC liposomes in this work. Results: The best mole ratio of DPPC-DOPE PEG2000, determined to be 50 to 1, was used to study the interaction with the polyclonal antibody AS25. The free energy of mixing (∆Gmix ) of DP-PC-DOPE PEG2000-AS25 was more negative than DPPC-AS25 in the entire investigated ranges, indicating that the ternary mixture of DPPC-DOPE PEG2000-AS25 was more compatible than the binary mixture of DPPC-AS25. The presence of DOPE PEG2000 in DPPC-AS25 increased the fluidity of the membrane, which resulted in a greater interaction of AS25 with DPPC. Conclusion: The constant values of particle size and zeta potential measurements of DPPC-DOPE PEG2000-AS25 liposomes showed agreement with the LB findings, indicating that LB is a good technique to predict precise liposomal formulations. © 2021.
Keywords
1, 2 dipalmitoyl sn glycero 3 phosphorylcholine, alcohol, antibody, drug, liposome, macrogol 2000, synaptosomal associated protein 25 antibody, unclassified drug, Article, atomic force microscopy, drug formulation, isotherm, Langmuir Blodgett film, nonhuman, particle size, transmission electron microscopy, zeta potential
Divisions
Science,CHEMISTRY
Publication Title
Current Chemical Biology
Volume
15
Issue
3
Publisher
Bentham Science Publishers