Isolation, screening and antibiotic sensitivity of pseudmoonas species from Kelana Jaya Lake Soil in Selangor Malaysia
Document Type
Article
Publication Date
1-1-2021
Abstract
Pathogenic microorganisms from hospitals, communities, and the environment remain great threats to human health. The increasing concern about antibiotic resistance has also necessitated the search for robust alternatives. Therefore, this study aims to isolate, screen and evaluate the antibiotic susceptibility of Pseudomonas aeruginosa isolated from a soil sample taken from northern, western and eastern parts of Kelana Jaya Lake against four antibiotics (gentamycin, tetracycline, ampicillin, and penicillin) on a Mueller-Hinton Agar media plate. Pseudomonas identification was done by using API 20 kit. Disc diffusion was employed as well as the oxidase test. From the positive oxidase result, the isolated bacteria were identified as Burkholderia cepacia (97.6 ID), Pseudomonas aeruginosa (99.5-99.9 ID), and Pseudomonas fluorescent (75.9 ID). Only Pseudomonas aeruginosa isolates were further evaluated for antibiotic susceptibility tests. The result showed that P. aeruginosa was susceptible to only three antibiotics (gentamycin, tetracycline, and penicillin) showing a clear zone of inhibition while it was resistant to only ampicillin with no zone of inhibition. Soil isolates are potential sources for the development of effective antibiotics against resistant bacteria. © 2021 University of Baghdad. All rights reserved.
Keywords
Pathogenic microorganisms, Antibiotics, Bacterial resistance
Divisions
cenar
Funders
None
Publication Title
Baghdad Science Journal
Volume
18
Issue
3
Publisher
Coll Science Women, Univ Baghdad