In vitro antioxidation, antihepatic steatosis, and anti-inflammatory effects of ethyl acetate fraction from Auricularia nigricans (Agaricomycetes)
Document Type
Article
Publication Date
1-1-2021
Abstract
Nonalcoholic fatty liver disease (NAFLD) is currently one of the most common liver diseases worldwide. Lifestyle modifications through the diet are the mainstay of treatment. Auricularia nigricans is a popular edible mushroom known to possess medicinal properties. Gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry analysis indicated that linoleic acid ethyl ester, butyl 9,12-octadecadienoate, 9,12-octadecadienoic acid, ergosta-5,7,22-trien-3-ol, 2(3,4-dihydroxyphenyl)-7-hydroxy-5-benzene propanoic acid, and 3,30-di-O-methyl ellagic acid were present in the A. nigricans ethyl acetate (EA) fraction. The cytotoxicity assay showed that the EA fraction was noncytotoxic to HepG2 cells at concentrations < 100 mu g/mL. In the antihepatic steatosis assay, 50 mu g/mL of EA fraction caused a decline in absorbance to 0.20 +/- 0.02 compared to palmitic acid (PA)-induced cells (0.24 +/- 0.02). Furthermore, cells treated with 50 mu g/mL and 25 mu g/mL of EA fraction contributed an approximately 1.12-fold and 1.08-fold decrease in lipid accumulation compared to PA-induced cells. Coincubation with PA and 25 mu g/mL of EA fraction decreased levels of tumor necrosis factor-alpha, interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 to 140.48 +/- 8.12, 91.16 +/- 2.40, 184.00 +/- 22.68, and 935.88 +/- 39.36 pg/mL compared to PA-induced cells. The presence of the EA fraction also suppressed the stress-activated protein kinase/Jun amino-terminal kinase, p-38 mitogen-activated protein kinase, nuclear factor-kappa B, and signal transducer and activator of transcription 3 signaling pathways. In conclusion, these findings suggest that the A. nigricans EA fraction demonstrates antisteatotic effects involving antioxidant capacity, hypolipidemic effects, and anti-inflammatory capacity in the PA-induced NAFLD pathological cell model.
Keywords
Auricularia nigricans, Nonalcoholic fatty liver disease (NAFLD), Palmitic acid-induced HepG2 cells, Medicinal mushrooms
Divisions
InstituteofBiologicalSciences
Funders
Universiti Malaya (PG142-2012B),HIR-MOHE grant (F00000221001)
Publication Title
International Journal of Medicinal Mushrooms
Volume
23
Issue
2
Publisher
Begell House Inc
Publisher Location
50 NORTH ST, DANBURY, CT 06810 USA