Detection of prostate cancer via IR spectroscopic analysis of urinary extracellular vesicles: a pilot study
Document Type
Article
Publication Date
1-1-2021
Abstract
Extracellular vesicles (EVs) are membranous nanoparticles naturally released from living cells which can be found in all types of body fluids. Recent studies found that cancer cells secreted EVs containing the unique set of biomolecules, which give rise to a distinctive absorbance spectrum representing its cancer type. In this study, we aimed to detect the medium EVs (200-300 nm) from the urine of prostate cancer patients using Fourier transform infrared (FTIR) spectroscopy and determine their association with cancer progression. EVs extracted from 53 urine samples from patients suspected of prostate cancer were analyzed and their FTIR spectra were preprocessed for analysis. Characterization of morphology, particle size and marker proteins confirmed that EVs were successfully isolated from urine samples. Principal component analysis (PCA) of the EV's spectra showed the model could discriminate prostate cancer with a sensitivity of 59% and a specificity of 81%. The area under curve (AUC) of FTIR PCA model for prostate cancer detection in the cases with 4-20 ng/mL PSA was 0.7, while the AUC for PSA alone was 0.437, suggesting the analysis of urinary EVs described in this study may offer a novel strategy for the development of a noninvasive additional test for prostate cancer screening.
Keywords
EVs, Exosomes, Prostate cancer, FTIR, Urine test, Diagnosis
Divisions
fac_med
Funders
Monash University Malaysia Large Strategic Research Grant (LG-2017-02-SCI),Fundamental Research Grant Scheme - Ministry of Higher Education Malaysia (FRGS/1/2019/SKK08/MUSM/02/4)
Publication Title
Membranes
Volume
11
Issue
8
Publisher
MDPI
Publisher Location
ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND