A sensitive reverse transcription loop-mediated isothermal amplification assay for direct visual detection of SARS-CoV-2
Document Type
Article
Publication Date
12-1-2020
Abstract
A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.
Keywords
Rapid detection
Divisions
fac_med,Parasit
Publication Title
American Journal of Tropical Medicine and Hygiene
Volume
103
Issue
6
Publisher
Mer Soc Trop Med & Hygiene
Publisher Location
8000 WESTPARK DR, STE 130, MCLEAN, VA 22101 USA