Antioxidants, toxicity, and nitric oxide inhibition properties of pyroligneous acid from Palm kernel shell biomass
Document Type
Article
Publication Date
11-1-2020
Abstract
Huge volumes of lignocellulosic biomass residues generated from agricultural activities such as oil palm biomass pose great environmental threats if improperly treated. In this study, pyroligneous acid (PA) obtained from slow pyrolysis of palm kernel shell (PKS) were evaluated for its antioxidant activity via chemical assays, toxicity, and potential as anti-inflammatory agent based on nitric oxide (NO) inhibition activity. The PA was extracted using ethyl acetate (EA) and fractionated using column chromatography. Fractions 13-17 that contain highest total phenolic contents (866.84 +/- 54.28 mu g GAE/mg) were chosen for subsequent studies. Results obtained were as follows; DPPH-75.34 +/- 3.40%, TEAC-1346.48 +/- 5.29 mu g Trolox/mg, FRAP-11.80 +/- 0.41 mmol Fe(II)/mg, hydroxyl radical scavenging-IC50 270.34 +/- 4.88 mu g/mL, superoxide radical scavenging-IC50 472.32 +/- 1.87 mu g/mL, cytotoxicity after 24 h at less than 50 mu g/mL-cell viability of >= 93.08% for RAW 264.7 macrophage cell and anti-inflammatory activity with NO production of 6.55% after 24 h at sample concentration of 25 mu g/mL. From the GC-MS analysis, phenols and derivatives were identified as major compound (83.24%) followed by esters (11.23%), and ketones (5.53%). The present of phenolic compounds namely benzene-1,2-diol, catechol (35.01%), 1,3-dimethoxy-2-hydroxybenzene, syringol (23.81%), and other catechol derivatives can be attributed to the antioxidant and anti-inflammatory activities determined. This study has successfully demonstrated the potential use of PA obtained from PKS as alternative antioxidant and anti-inflammatory agent. GRAPHICS]
Keywords
Pyroligneous acid, Palm kernel shell, Phenolic, Antioxidants, Anti-inflammatory
Divisions
fac_med
Funders
Universiti Teknologi Malaysia (UTM) (07G78),Ministry of Education, Malaysia (4F994)
Publication Title
Waste and Biomass Valorization
Volume
11
Issue
11
Publisher
Springer Netherlands
Publisher Location
VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS