Expression of cytosolic and thiolated proteome of Musca domestica larvae under oxidative challenge
Document Type
Article
Publication Date
9-1-2020
Abstract
The study was aimed to investigate the expression of cytosolic and thiolated proteins of Musca domestica larvae under oxidative stress. Proteins from acute treatment of hydrogen peroxide (LC50 = 21.52% (v/v)) on 3rd stage larvae of housefly were extracted and purified using an activated Thiol Sepharose (R) for thiolated protein purification. Two dimensional gel electrophoresis was used for visualizing and analyzing expression of cytosolic and thiolated proteins. Protein spots with more than 5 fold of expression change were identified using liquid chromatography- tandem mass spectrometry (LC-MS/MS). The cytosolic proteins were actin, tropomyosin, ubiquitin, arginine kinase, pheromone binding protein/general odorant binding protein, and ATP: guanidino phosphotransferase. The thiolated proteins with more than 5 fold change in expression as an effect to the acute treatment were fructose bisphosphate aldolase, short chain dehydrogenase and lactate/malate dehydrogenase. The proteins identified in the study should provide vital information for future reference in oxidative stress defence and response occurring in houseflies.
Keywords
Animals, Cytosol, Houseflies, Larva, Lipid Peroxidation, Oxidative Stress, Proteome
Divisions
InstituteofBiologicalSciences
Funders
Ministry of Higher Education [Grant No: FP052-2014A],University of Malaya Postgraduate Research Fund [Grant No: PPP: PV091/2011A)]
Publication Title
Tropical Biomedicine
Volume
37
Issue
3
Publisher
Malaysian Society for Parasitology
Publisher Location
C/O INST MEDICAL REEARCH, JALAN PAHANG, KUALA LUMPUR, 50588, MALAYSIA